Our BPC-157 Just Tested at 99.8% Purity — Here’s What That Means
We test every batch. Not some batches. Not the first batch and then coast on it for six months. Every single batch of every peptide we sell goes to an independent, third-party analytical laboratory before it ever reaches our catalog.
Our latest batch of BPC-157 just came back from Janoshik Analytical at 99.8% purity via HPLC analysis.
If you’re a researcher sourcing peptides, that number matters. If you’re not sure why, this article will break it all down — what 99.8% actually means, how the testing works, why we use Janoshik, and how to read a Certificate of Analysis (COA) so you can evaluate any vendor’s claims for yourself.
What 99.8% Purity Actually Means
When we say our BPC-157 tested at 99.8% purity, we’re referring to a specific analytical measurement: High-Performance Liquid Chromatography, or HPLC. This isn’t a marketing number or a rounded estimate. It’s the result of a precise laboratory instrument separating and quantifying every molecular component in the sample.
How HPLC Works
HPLC is the gold standard for peptide purity assessment. The technique works by dissolving the peptide sample in a solvent and pushing it through a column packed with specialized material — typically a C18 reversed-phase column. Different molecules interact with the column material differently, causing them to elute (come out) at different times. A UV detector measures what comes through, producing a chromatogram — essentially a graph of peaks, where each peak represents a different molecular species in the sample.
The target peptide appears as the dominant peak. Purity is calculated by comparing the area of the target peak to the total area of all detected peaks. When we report 99.8% purity, it means that 99.8% of all UV-absorbing material in the sample is confirmed BPC-157. The remaining 0.2% constitutes minor impurities [1].
What’s in the Other 0.2%?
In peptide synthesis, trace impurities are expected and well-understood. The 0.2% typically consists of:
- Truncated sequences — shorter peptide fragments where the synthesis terminated one or two amino acids early
- Deletion sequences — variants where a single amino acid was skipped during solid-phase synthesis
- Counter-ion salts — residual trifluoroacetic acid (TFA) or acetate salts from the purification process
- Oxidation products — minor modifications from exposure to air during handling
None of these are toxic contaminants or foreign substances. They’re predictable byproducts of peptide manufacturing. But the lower that number, the cleaner your research compound — and 99.8% is exceptional by any standard in the industry.
Putting 99.8% in Context
Most research-grade peptides on the market fall between 95-98% purity. Anything above 98% is considered high purity. Above 99% is premium. At 99.8%, we’re operating at the upper boundary of what solid-phase peptide synthesis can reliably deliver for a 15-amino-acid sequence like BPC-157. Published research on BPC-157 — a stable gastric pentadecapeptide with the sequence Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val — has generated significant scientific interest across multiple areas of study [2][3].
Why Janoshik Analytical
Choosing which lab performs your testing matters as much as the testing itself. We use Janoshik Analytical, an independent laboratory based in the Czech Republic that has become the recognized standard for peptide and research compound analysis.
Here’s why we chose them — and why we’ve never switched:
True Independence
Janoshik has no financial relationship with any vendor. They don’t sell peptides. They don’t have affiliate agreements. They don’t offer discounts for favorable results. Their sole business is analytical testing. When a result comes back from Janoshik, it reflects what’s in the vial — period. This separation between testing and commerce is fundamental to the integrity of any Certificate of Analysis.
Specialized Instrumentation
Janoshik employs HPLC systems and mass spectrometry (MS) equipment specifically calibrated for peptide analysis. Peptides present unique analytical challenges compared to small molecules — they’re larger, more complex, and their impurity profiles require expertise to properly interpret. A general chemistry lab running the same HPLC column they use for pharmaceutical small molecules won’t deliver the same level of accuracy for a pentadecapeptide like BPC-157.
Public Accountability
Janoshik maintains a public database of test results at public.janoshik.com. This means results can be verified. It’s one thing for a vendor to upload a PDF of a COA — it’s another when that COA is traceable to an independent, publicly accessible database. Transparency isn’t just a company value statement when the data lives on someone else’s server.
What a Certificate of Analysis Actually Tells You
A COA is the document that accompanies analytical testing. For peptide research compounds, a thorough COA should include four key components:
1. Identity Confirmation (Mass Spectrometry)
Before you can assess purity, you need to confirm you’re looking at the right molecule. Mass spectrometry (MS) measures the molecular weight of the compound and compares it to the known theoretical mass. For BPC-157, the expected molecular weight is approximately 1419.53 Da (daltons). If the MS result doesn’t match within an acceptable tolerance, the compound isn’t what it claims to be — regardless of what the HPLC says about purity. Identity testing is the first gate, and it’s non-negotiable [1].
2. Purity Assessment (HPLC)
This is the headline number — the percentage of the target peptide relative to total detected material. As described above, HPLC separates the sample into its molecular components and quantifies each one. For research-grade peptides, you want to see this number at 98% or higher. Our 99.8% result means the synthesis, purification, and handling of this batch were all executed at an extremely high level.
3. Endotoxin Testing
Endotoxins are lipopolysaccharide fragments from bacterial cell walls. They can be present as contaminants in any manufactured compound, and they’re a significant concern in laboratory research because they trigger immune responses in biological systems — which can compromise experimental results. Endotoxin testing, typically via the Limulus Amebocyte Lysate (LAL) assay, ensures levels are below acceptable thresholds, usually reported in Endotoxin Units per milligram (EU/mg).
4. Sterility Testing
For lyophilized (freeze-dried) peptides, sterility testing confirms the absence of viable microbial contamination. This is critical for researchers working with cell cultures or in vitro systems where microbial contamination would invalidate results.
How to Read a COA: A Quick Guide
Not all COAs are created equal, and knowing what to look for separates informed researchers from those taking vendor claims at face value. Here’s what to check:
The Numbers That Matter
Molecular weight (MS): Should match the target peptide’s theoretical mass. For BPC-157, look for ~1419.53 Da. If this is off by more than ±1 Da, question the identity of the compound.
HPLC purity (%): The higher the better. Below 95% is concerning for research applications. 98%+ is solid. 99%+ is excellent.
HPLC chromatogram: A legitimate COA should include the actual chromatogram — the graph showing peaks. You should see one dominant peak (your target peptide) with minimal noise. If the chromatogram is missing, that’s a red flag.
Endotoxin level: Should be reported as <1.0 EU/mg for high-quality research compounds. Some vendors skip this entirely.
Lab identification: The COA should clearly state which lab performed the analysis, include a unique test ID or batch reference, and ideally be traceable to a public database.
Red Flags to Watch For
- No chromatogram included. If a vendor gives you a purity number with no supporting data, you’re taking their word for it.
- In-house testing only. A COA from the same company selling the product isn’t independent verification — it’s a claim.
- Suspiciously round numbers. Real HPLC results come back as 99.23% or 98.71%, not always a clean “99%.”
- Reused COAs. If the same document appears across multiple product pages with different batch numbers but identical chromatograms, something is wrong.
- Missing batch or lot numbers. A COA without a traceable batch reference can’t be tied to the specific product you’re purchasing.
Why Third-Party Testing Isn’t Universal
If independent third-party testing is so important, why don’t all peptide vendors do it? Three reasons:
Cost
Independent analytical testing isn’t cheap. A comprehensive peptide analysis — HPLC purity, mass spec identity, endotoxin, sterility — costs real money per batch. When you’re testing every batch of every product, those costs add up fast. Comprehensive testing of every batch requires treating quality verification as an ongoing operational commitment, not a one-time checkbox.
Risk
When you send a sample to an independent lab, you can’t control the result. If a batch comes back below spec, you own that data — and you make the call not to sell it. We believe that accountability is worth the cost, because credible research depends on verified materials.
Accountability
Publishing real COAs from an independent lab creates a public record. Researchers can compare batches over time, quality consistency becomes demonstrable, and the data speaks for itself. That level of accountability is exactly what we believe the research peptide market needs.
Our Commitment: Every Batch, Every Product, Every Time
At Chameleon Peptides, third-party testing isn’t a marketing tactic — it’s an operational requirement. Here’s what that means in practice:
- Every batch of every peptide is sent to Janoshik Analytical before release
- Full COAs — including chromatograms — are published and accessible
- Results are unedited — we publish what comes back, as it comes back
- Batch numbers are traceable — every COA ties to a specific production lot
- Sub-standard batches don’t ship — if a batch doesn’t meet our purity threshold, it gets rejected. Period.
We do this because we believe transparency should be the standard in the research peptide market. Researchers deserve verifiable, independent, reproducible data — and that’s what we provide with every batch.
BPC-157 is one of the most widely studied peptides in the published literature, with extensive research spanning multiple experimental models [2][3][4][5]. Researchers working with this compound need to know that the material in their hands matches the material described in published studies. A COA from an independent lab is the closest thing to that guarantee.
See the Results for Yourself
Every batch result is publicly available. The data speaks for itself.
👉 View all of our third-party lab results and COAs
👉 View our BPC-157 product page
Every COA on our testing page is a real document from Janoshik Analytical, linked to a specific batch, showing real chromatograms and real numbers. Browse them. Compare them. Hold us to them.
That’s our standard — and we stand behind it.
References
- Aguilar MI. “HPLC of Peptides and Proteins.” Methods in Molecular Biology, vol. 251, 2004. DOI: 10.1385/1-59259-742-4:3. PMC7119934
- Sikiric P, et al. “Stable Gastric Pentadecapeptide BPC 157 and Wound Healing.” Frontiers in Pharmacology, 2021;12:627533. DOI: 10.3389/fphar.2021.627533. PMID: 34248616
- Sikiric P, et al. “Brain-gut Axis and Pentadecapeptide BPC 157: Theoretical and Practical Implications.” Current Neuropharmacology, 2016;14(8):857-865. DOI: 10.2174/1570159X14666160708224518. PMID: 27306034
- Chang CH, et al. “Pentadecapeptide BPC 157 Enhances the Growth Hormone Receptor Expression in Tendon Fibroblasts.” Molecules, 2014;19(12):19066-77. DOI: 10.3390/molecules191119066. PMID: 25415479
- Sikiric P, et al. “Stable Gastric Pentadecapeptide BPC 157 and Striated, Smooth, and Heart Muscle.” Current Pharmaceutical Design, 2023;29(7):519-538. DOI: 10.2174/1381612829666221222154329. PMID: 36551977